Amylases in Developing Barley Seeds
نویسندگان
چکیده
منابع مشابه
Amylases from aleurone layers and starchy endosperm of barley seeds.
Amylases from incubated aleurone layers or from starchy endosperm of barley seeds (Hordeum vulgare L. cv. Himalaya) were investigated using acrylamide gel electrophoresis and analytical gel filtration with Sephadex G-200. Electrophoresis of amylase from aleurone layers yields seven visually distinct isozymes with an estimated molecular weight of 43,000. Because each isozyme hydrolyzes beta-limi...
متن کاملSearch for endophytic diazotrophs in barley seeds
Eight endophytic isolates assigned to Pseudomonas, Azospirillum, and Bacillus genera according to pheno-genotypic features were retrieved from barley seeds under selective pressure for nitrogen-fixers. Genetic relationships among related isolates were investigated through RAPD. Six isolates displayed nitrogen-fixing ability, while all could biosynthesize indolacetic acid in vitro and showed no ...
متن کاملMetabolic Regulation in Developing Barley Seeds – Novel Insights from Transcriptome Analyses
The barley (Hordeum vulgare) seed, termed caryopsis, is of high importance for feed and food industry. In addition, it provides an excellent model to study the development and physiology of starch-storing cereal seeds. Gene expression in caryopses, like all plant organs, is to a large extent regulated by the cellular sugar status. As part of the sugar signalling cascade, we have previously iden...
متن کاملSubcellular analysis of starch metabolism in developing barley seeds using a non-aqueous fractionation method
Compartmentation of metabolism in developing seeds is poorly understood due to the lack of data on metabolite distributions at the subcellular level. In this report, a non-aqueous fractionation method is described that allows subcellular concentrations of metabolites in developing barley endosperm to be calculated. (i) Analysis of subcellular volumes in developing endosperm using micrographs sh...
متن کاملalpha-Amylase Isozymes in Gibberellic Acid-treated Barley Half-seeds.
The presence of multiple forms of alpha-amylase in gibberellic acid-treated embryoless barley half-seeds was demonstrated by separation on diethylaminoethyl-Sephadex and isoelectric focusing polyacrylamide gel disc electrophoresis. Two major alpha-amylase fractions (A and B), each consisting of two to three isozyme components, were purified. alpha-Amylase fractions A and B were distinguishable ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1971
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.48.3.331